Journal: Molecular Medicine Reports

Article Title: High expression of B23 is associated with tumorigenesis and poor prognosis in bladder urothelial carcinoma

doi: 10.3892/mmr.2016.6033

Figure Lengend Snippet: Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by immunohistochemistry analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.

Article Snippet: Non-specific antigen-antibody reactions were inhibited using an immunohistochemistry Protein Blocker-serum and Azide Free (MB-071-0100, Rockland Immunochemicals, Inc., Pottstown, PA, USA) for 5 min, following which the slides were washed thoroughly with PBS.

Techniques: Expressing, Immunohistochemical staining, Staining, Immunohistochemistry, Western Blot, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction